Product details
| Organism | Homo sapiens |
| Source | Cerebral microvessel |
| Morphology | Spindle-shaped, elongated |
| Recommended Medium | EBM-2 Endothelial basal medium (Lonza, #190860)
Supplemented with:
- Fetal Bovine Serum 5%
- Chemically Defined Lipid Concentrate (Life technologies, # 11905031): 1/100
- Ascorbic Acid (Sigma, #A4544) - 5µg/mL
- bFGF: human Basic Fibroblast Growth Factor, (Sigma, #F0291) - 1ng/mL
- HEPES: 10mM
- Hydrocortisone (Sigma, #H0135): 1.4µM |
| Freeze Media | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Biosafety Level | 2 |
| Storage and Shipping | The frozen cells will be shipped with dry ice, and should be stored in liquid nitrogen immediately. |