Product details
| Source | HEK293 Cells |
| Species | Rat |
| Bio-activity | Measured by its ability to inhibit human MMP2 cleavage of a fluorogenic peptide substrate MCA-PLGLDPA-AR-NH2, AnaSpec Catalog # 27076.
The IC50 value is approximately 4 nM. |
| Length | A DNA sequence encoding the rat TIMP1 (P01860) (Ser2-Ala217) was expressed and purified. |
| Molecular Mass | The recombinant rat TIMP1 consists of 194 amino acids and has a predicted molecular mass of 21.5 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 28 kDa in SDS-PAGE under reducing conditions. |
| Purity | > 97 % as determined by SDS-PAGE |
| Endotoxin | Endotoxin: ＜1.0 EU per μg by the LAL method. |
| Buffer | Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 7.0. |
| Storage | Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution. |
| Shipping | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |